Contact an Account ManagerMeasurement of vitamin K in human liver by gradient elution HPLC using platinum black catalyst reduction and fluorimetric detection
Summary
Type: HPLC
Application #: 109730
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence after postcolumn reduction with platinum black powder, excitation 320nm, emission 430nm
Conditions: eluent A:
methanol
eluent B:
2-propanol - ethanol (4:1, v/v)
15min isocratic eluent A, then linear gradient from 0 to 80% B in 75min
flow rate 1ml/min
Authors: Usui Y
Source: J Chromatogr 489 (1989) 291 - 301
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